Additional information
Brand | Other Labs |
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£120.00
Recombinant Human Growth hormone (hGH)
Source:
A DNA sequence encoding the mature hGH protein was expressed in P. Pastoris
Formulation:
0.2 μm-filtered solution in 20mM glycine, 200mM Mannitol, 20mM NaH2PO4 , pH 7.4; m-cresol 2mg/ml, glycerol 4 mg/ml
Protein concentration per 1ml:
3.3 mg ±4%
Release Testing: | Specification | Lot Result |
---|---|---|
Specific Activity | 3.4 IU/mg | 3.0-5 IU/mg |
Purity: | ≥ 97% | >98.5% |
Identity: | Complies | Complies |
Sterility: | Sterile | Complies |
Endotoxin level: | <20 EU/mg | <10 EU/mg |
Host-cell DNA: | ≤ 200ng/mg | Complies (1.1ng/mg) |
Activity was determined using a Nb2-11 cell proliferation assay
Purity was determined by SDS PAGE and coomassie stain (under reducing and non-reducing conditions). Identity was confirmed by end-of-production DNA sequencing and N-terminal protein sequencing. Sterility test of vialed product was performed according to Eur.Pharm. (Inoculation method).
Endotoxin was determined using the gel clot assay according to Eur.Pharm.
Host-cell DNA was determined using fluorimetric assay.
General usage:
Open cap, clean the rubber stopper with disinfectant napkin or other cleaning disinfection method / material. Puncture rubber stopper with sterile needle and draw the ready to use product (see below) into sterile syringe according doctor instructions.
Using liquid product:
Liquid product is ready to use according doctor instructions
Dilution for ex vivo procedures:
Recommended in CellGro®/CellGenixTM serum free media. For dilution with PBS or protein free medium a carrier protein (0.1-1% albumin or 1-10% appropriate serum) has to be included. Failure to dilute product according to these instructions will result in loss of activity.
Storage and stability:
Store material at +4 – +8C0, do not freeze. Store diluted material for ex vivo use at +40C. Do not freeze!
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